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taining solid particles Both NML Cyg and VY CMa display circular polarization in the infrared as described by Serkowski (1973) Maximum v occurs near the wavelength of minimum linear polarization, as expected from Mie scattering theory VY CMa may be classi ed as a hypergiant Being the largest known star, and also one of the most luminous, it has attracted much observational attention as an OH emission source Large polarization was detected by both Shawl (1969) and Serkowski (1969a), together with a strong wavelength dependence of the position angle The UBV measurements suggested that Rayleigh scattering by circumstellar dust grains produces the polarization, rather than synchrotron radiation being present Hashimoto, Maihara, Okuda, et al (1970) made observations of this object in the infrared H- and K-bands nding the values to follow an extrapolation of measurements made previously by others in the visual part of the spectrum Forbes (1971) extended the polarization measurements of VY CMa into the infrared, con rming a near-linear rise of p with wavenumber Extremely high values of polarization in the blue spectrum region have been observed by Serkowski (1969b) in the nebulae associated with VY CMa and R CrA For the former object, the value of p was 40% Both stars also showed signi cant changes in p over an interval of a few weeks By exploring the polarization structure associated with the nebulosity surrounding VY CMa, Herbig (1972) suggested that the temporal variability of the integrated radiation can be explained by the photometric variability of the star, or of portions of the nebula The dispersion of the polarization position angle can be explained by a wavelength variation of the relative brightness of the star and various parts of the nebula Remarkable structure in the spectral range 4000 8250 , with reductions in p 1% and rotations of direction of vibration 10 across the TiO bands, have been recorded by Aspin, Schwarz & McLean (1985) for VY CMa Using a cell hotspot model , they reproduced the characteristics of the observed behaviour, providing possible values for the sizes of the hotspots and their temperatures The difference in behaviour between VY CMa and Ori can be accounted for by an increase in the amount of scattering material, or by taking the effective temperature as 2700 K, compared with 3900 K The Becklin Neugebauer (BN) object in the Orion nebula has also attracted polarimetric attention Breger & Herdorp (1973) made observations at 16 m and found a polarization of 25% In addition, they also investigated NU Ori, also near to the Orion Nebula, with a range of passbands, and noticed that there was a signi cant rotation of the position angle across the spectrum Loer, Allen & Dyck (1973) extended the infrared polarization measurements of the BN object to 22 and 35 m The polarization displayed a minimum between 4 and 8 m encouraging circular polarization measurements to be attempted by Serkowski & Rieke (1973) with positive results Some part of the polarization appears to have its source in a dense cloud, with mean alignment of particles changing along the line of sight Dyck, Capps, Forrest, et al (1973) found this same object to be ighly polarized in the 5 11 m spectral range From 8 to 11 m, the polarization is strongly correlated with the optical depth in the 10 m absorption feature, and reaches a maximum of about 15% at 10 m The observations are consistent with the polarization being.

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These methods allowed MS to be introduced to the biological chemistry area, and re now particularly useful for the study of proteins, glycoproteins, nucleic acids, and their reactions. Molecular weights are now readily determined for these molecules with very high precision and accuracy. Variation between the experimental and the predicted MW of a protein (predicted from its DNA code) help in the determination of posttranslational modi cations of the polypeptide chain. Noncovalent interactions between biomolecules and ligands may also be studied using soft ionization methods. Mass sepetrometry has also been used to analyze nucleic acid mutations and polymorphisms, and for the identi cation and chemical characterization of microbial cells. 15.2. BASIC DESCRIPTION OF THE INSTRUMENTATION As shown in Figure 15.1, there are three main components of every mass spectrometer. The ion source is used to produce gas-phase ions by capture or loss of electrons or protons. In the mass analyzer, the ions are separated according to their m/z ratios: ions of a particular m/z value reach the detector, and a current signal is produced. This section describes the soft ionization sources, mass analyzers, and detectors that are used in experiments involving biological macromolecules. . Draw Barcode In Visual Basic .NET Using Barcode generator for .NET Control to .Related: PDF417 Generating Java , PDF417 Generating .NET , Create Code 128 Java

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